University of California, Riverside

School of Medicine

Faculty Biographies

David A. Johnson

Professor Emeritus of Biomedical Sciences

David A. Johnson

University of California, Riverside
Riverside, CA 92521

Tel: (909) 335-8168

Education and Training

  • Ph.D., University of California, San Francisco, California

Research Interests

  • Molecular pharmacology.
  • Molecular basis of the functional regulation of the nicotinic acetylcholine receptor, cAMP-dependent protein kinase, Pak2, AMP-activated protein kinase, and acetylcholinesterase.
  • Spectroscopic approaches to analyze the molecular details of drug-receptor interactions, especially with reference to the nicotinic receptor, cAMP-dependent protein kinase, and acetylcholinesterase.
  • Molecular dynamics of the cAMP-dependent protein kinase, acetylcholinesterase, acetylcholine binding protein, and Pak2.
  • Molecular basis for hemodynamic induced atherosclerosis susceptibility.

Selected Publications

  • Marin TL, Gongol B, Martin M, King SJ, Smith L, Johnson DA, Subramaniam S, Chien S, Shyy JY. Identification of AMP-activated protein kinase targets by a consensus sequence search of the proteome. BMC Syst Biol. 2015; 9(1):13.
  • Chen Z, Wen L, Martin M, Hsu CY, Fang L, Lin FM, Lin TY, Geary MJ, Geary GG, Zhao Y, Johnson DA, Chen JW, Lin SJ, Chien S, Huang HD, Miller YI, Huang PH, Shyy JY. Oxidative Stress Activates Endothelial Innate Immunity via Sterol Regulatory Element Binding Protein 2 (SREBP2) Transactivation of MicroRNA-92a. Circulation. 2015 Mar 3; 131(9):805-14.
  • Gongol B, Marin T, Peng IC, Woo B, Martin M, King S, Sun W, Johnson DA, Chien S, Shyy JY. AMPKa2 exerts its anti-inflammatory effects through PARP-1 and Bcl-6. Proc Natl Acad Sci U S A. 2013 Feb 19; 110(8):3161-6.
  • Chen Z, Shentu TP, Wen L, Johnson DA, Shyy JY. Regulation of SIRT1 by oxidative stress-responsive miRNAs and a systematic approach to identify its role in the endothelium. Antioxid Redox Signal. 2013 Nov 1; 19(13):1522-38.
  • Wen L, Chen Z, Zhang F, Cui X, Sun W, Geary GG, Wang Y, Johnson DA, Zhu Y, Chien S, Shyy JY. Ca2+/calmodulin-dependent protein kinase kinase ß phosphorylation of Sirtuin 1 in endothelium is atheroprotective. Proc Natl Acad Sci U S A. 2013 Jun 25; 110(26):E2420-7.
  • Xiao H, Lu M, Lin TY, Chen Z, Chen G, Wang WC, Marin T, Shentu TP, Wen L, Gongol B, Sun W, Liang X, Chen J, Huang HD, Pedra JH, Johnson DA, Shyy JY. Sterol regulatory element binding protein 2 activation of NLRP3 inflammasome in endothelium mediates hemodynamic-induced atherosclerosis susceptibility. Circulation. 2013 Aug 6; 128(6):632-42.
  • Bastidas, A.C., Pierce, L.C., Walker, R.C., Johnson, D.A., Taylor, S.S., "Influence of N-Myristylation and Ligand Binding on the Flexibility of the Catalytic Subunit of Protein Kinase A", Biochemistry, 2013, 52 (37) 6368-6379.
  • Peng, I.-C., Chen, Z., Sun, W., Li, Y.-S., Marin, T. L., Hsu, P.H., Hsu, P.-H., Su, M.-I., Cui, X., Pan, S., Lytle, C. Y., Johnson, D. A., Blaeser, F., Chatila, T., and Shyy, J. Y.-J. 2012. Glucagon Regulates ACC Activity in Adipocytes Through the CaMKKβ/AMPK Pathway. J Physiol Endocrinol Metab. 2012 Jun 15; 302(12):E1560-8.
  • Anand, G. A., Krishnamurthy, S., Bishnoi, T., Kornev, A., Taylor, S. S., and Johnson, D. A. 2010. Cyclic-AMP and Rp-cAMPS Induced Conformational Changes in a Complex of the Catalytic and Regulatory (RIα) Subunits of PKA. Molecular and Cellular Proteomics, 10, 2225-37.
  • Hsu, Y.H., Johnson, D.A., and Traugh, J.A. 2008. Analysis of Conformational Changes during Activation of Protein Kinase Pak2 by Amide Hydrogen/Deuterium Exchange. J. Biol. Chem. 283, 36397-36405.
  • Anand, G., Taylor, S.S., and Johnson, D.A. 2007. Cyclic-AMP and Pseudosubstrate Effects on A-Kinase Regulatory and Catalytic Subunit Binding Kinetics. Biochemistry, 46, 9283-9291.
  • Hibbs, R.E., Radic, Z., Palmer Taylor, P., and Johnson, D.A. 2006 Influence of Agonists and Antagonists on the Segmental Motion of Residues near the Agonist Binding Pocket of the Acetylcholine-binding Protein. J. Biol. Chem. 281, 39708 – 39718.
  • Hibbs, R. E., Johnson, D. A., Shi, J., Hansen, S. B., and Taylor, P. 2005. Structural Dynamics of The A-Neurotoxin-Acetylcholine Binding Protein Complex: Hydrodynamic and Fluorescence Anisotropy Decay Analyses, Biochemistry 44, 16602-16611.
  • Tan, D., Johnson, D. A., Wu, W., Zeng, W., Chen, Y. H., Vonderhaar, B. K., & Walker, A. W. 2005 Prolactin (PRL) and S179D PRL-initiated Bioluminescence Resonance Energy Transfer Between Homo- and Hetero-pairs of Long and Short Human Prolactin Receptors in Living Human Cells. Molecular Endocrinology (in press).
  • Yang, S., Rogers, K. M., and Johnson, D. A. 2005 MgATP-induced conformational change of the catalytic subunit of cAMP-dependent protein kinase. Biophysical Chemistry 113, 93-99.
  • Boyd, A. E., Dunlop, C. S., Marnett, A. B., Wong, L., Taylor, P., and Johnson, D. A. 2004 Nanosecond Dynamics of the Mouse Acetylcholinesterase near the Active-Site Gorge, J. Biol. Chem. 279: 26612-26618.
  • Zawadzki, K. M., Chia-Pin Pan, C.-P., Johnson, D.A. Barkley, M.D., Taylor, S.S. 2003 Endogenous Tryptophan Residues of cAPK Regulatory Subunit Type IIβ Reveal Local Variations in Chemical Environments and Dynamic States, 2003 Proteins: Structure, Function and Genetics 51: 552-561.
  • Shi, J., Tai, K., McCammon, J.A., Taylor, P., and Johnson, D. A. 2003 Nanosecond Dynamics of the Mouse Acetylcholinesterase Cys69-Cys96 Omega Loop, 2003 J. Biol. Chem. 33: 30905-30911.
  • Li, F., Gangal, M., Juliano, C., Gorfain, E., Taylor, S.S. & Johnson, D.A. 2002. Evidence for an internal entropy contribution to phosphoryl transfer: a study of domain closure, backbone flexibility, and the catalytic cycle of cAMP-dependent protein kinase. J. Mol. Biol. 315: 459-469.
  • Johnson, D.A., Akamine, P., Radzio-Andzelm, E., Madhusudan, M. & Taylor S.S. 2001. Dynamics of cAMP-Dependent Protein Kinase. Chem. Rev. 101: 2243-2270.
  • Li, F., Gangal, M., Jones, J.M., Deich, J., Lovett, K.E., Taylor, S.S. & Johnson, D.A. 2000. Consequences of cAMP and Catalytic-Subunit Binding on the Flexibility of the A-Kinase Regulatory Subunit. Biochemistry 39: 15626-15632.
  • Garzon-Rodriguez, W., Vega, A., Sepulveda-Becerra, M., Milton, S., Johnson, D.A., Yatsmirsky, A.K. & Glabe, G. 2000. A Conformation Change in the Carboxyl-terminus of Alzheimer's Ab(1-40) Accompanies the Transition from Dimer to Fibril as Revealed by Fluorescence Quenching Analysis. J. Biol. Chem. 275: 22645-22649.
  • Gangal, M., Clifford, T., Deich, J., Cheng, X., Taylor, S.S. & Johnson, D.A. 1999. Mobilization of the A-Kinase through an Isoform-Specific Intermolecular Switch. PNAS 96, 12394-12399.
  • Johnson, D. A., Nguyen, B., Bohorquez, A. F., & Valenzuela, C. F. 1999. Paramagnetic Fluorescence Quenching in a Model Membrane: A Consideration of Lifetime and Temperature. Biophysical Chemistry, 77, 1-9.
  • Hauer, J. A. Taylor, S. S., & Johnson, D.A. 1999. Binding-Dependent Disorder-Order Transition in PKI-alpha: A Fluorescence Anisotropy Study. Biochemistry, 38, 6774-6780.
  • Gangal, M., Cox, S., Lew, J., Clifford, T., Garrod, S. M., Aschbaher, M.,Taylor, S. S., and Johnson, D. A. 1998. Backbone Flexibility of Five Sites on the Catalytic Subunit of cAMP-Dependent Protein Kinase in the Open and Closed Conformations. Biochemistry 37, 13728-13735.
  • Johnson, D. A. and Ayres, S. 1996. Quinacrine noncompetitive inhibitor binding site localized on the nicotinic acetylcholine receptor in the open state. Biochemistry 35, 6330-6336.
  • Yang, S., Fletcher, W. H., and Johnson, D. A., 1995. Regulation of cAMP-dependent protein kinase: Enzyme activation without dissociation. Biochemistry 34, 6267-6271.
  • Johnson, D.A. and Nuss, J., 1994. The phencyclidine-sensitive ethidium binding site is located outside the transmembrane domain of the nicotinic acetylcholine receptor: a fluorescence study. Biochemistry 33, 9070-9077.
  • Valenzuela, C. F., Dowling, A. J., Arias, H.R., and Johnson, D.A., 1994. Antibody-induced conformational changes in the Torpedo acetylcholine receptor: a fluorescence study. Biochemistry 33, 6586-6594.
  • Valenzuela, C. F., Wiegn, P., Yguerabide, J., and Johnson, D.A. 1994. Transverse distance between the agonist binding sites and the membrane on the Torpedo acetylcholine receptor: a fluorescence study. Biophys. J. 66, 674-682.

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